Food Allergens

An allergen is a substance usually harmless to most people, but in some individuals (those with allergies) it can produce allergic reactions of various kinds, sometimes even very serious.

Allergen typologies

Neotron is able to determine the allergens listed in Annex II of EU regulation 1169/2011 on different types of matrices. Other allergens included in other non-EU countries specific regulations are also determined.

Analytical techniques

The most known and commonly used techniques are Enzyme-linked immunosorbent assay (E.L.I.S.A.) and Real time PCR (Polymerase Chain Reaction), which target respectively proteins and DNA. The analytical package for allergen detection is completed by mass spectrometry (LC-MS / MS), an instrumental technique that plays a fundamental role in confirming with E.L.I.S.A. and RT PCR results.

Digital PCR is an evolution of Real Time PCR that Neotron today proposes in an innovative way for the detection and quantification of allergens’ DNA. With this method it is possible to perform absolute quantifications of the target, reach lower detection limits, reduce the effect of potential inhibitors present in the matrices with a very high level of reproducibility.
The complete analytical portfolio can be requested from the technical sales office (


Accreditation by Accredia (Italian Accreditation Body) according to UNI CEI EN ISO/IEC 17025 standard.

Laboratory authorized by the Italian Celiac Association (AIC) for the analytical determination of gluten both with the sandwich and competitive methods. (First emission: 2002)
Laboratory authorized by the Federación de Asociaciones de Celiacos de España (FACE) for the analytical determination of gluten. (First emission: 2006)
The systematic involvement in Proficiency Test provides the external evidence about the test methods applied.
The results relating to the Proficiency Tests performed in the last year are available and can be requested from the Quality Assurance  department.

Main reference legislation for allergens

Regulation (EU) No 1169/2011 of the European Parliament and of the Council of 25 October 2011 on the provision of food information to consumers, amending Regulations (EC) No 1924/2006 and (EC) No 1925/2006 of the European Parliament and of the Council, and repealing Commission Directive 87/250/EEC, Council Directive 90/496/EEC, Commission Directive 1999/10/EC, Directive 2000/13/EC of the European Parliament and of the Council, Commission Directives 2002/67/EC and 2008/5/EC and Commission Regulation (EC) No 608/2004.

Latest update: 22/09/2021 17:01:11